Fig. 6

EphB2 promotes NF-κB-dependent pro-inflammatory activation of astrocytes through activation of Erk and p38-MAPK signaling cascade. a Astrocytes isolated from brains of neonatal WT mice were treated with 10 nmol pre-clustered EphB2/Fc, anti-IgG Fc or 30 nmol rmTNF for 6 h. Immunofluorescent staining was used to determine the nuclear accumulation of NF-κB (mean ± SD; n = 3; Student’s t-test). The top-right panel shows representative immunofluorescent staining images: NF-κB (red) and nuclei (blue). b-d Astrocytes were treated with either (b) 10 μM Bay 11–7082, (c) 20 μM PD98059 or (d) 10 μM SB203580 for 1 h prior to stimulation with pre-clustered EphB2/Fc or anti-IgG Fc for 6 h. Gene expression was analyzed by quantitative real-time RT-PCR (mean ± SD; n = 3 (Bay 11–7082), n = 3 (PD98059), n = 4 (SB203580); One-way ANOVA with Holm-Sidak’s multiple comparisons test). * p < 0.05