Fig. 5
EphB2/ephrin-B reverse signaling promotes pro-inflammatory activation of astrocytes. a Primary microglia isolated from brains of neonatal WT mice were exposed to normoxic or OGD conditions for 6 h in the presence of either 10 nmol pre-clustered EphB2/Fc or anti-IgG Fc. Gene expression was determined by quantitative real-time RT-PCR (mean ± SD; n = 5 (N), n = 4 (OGD); Student’s t-test). b Primary astrocytes derived from brains of new born WT and nEfnb2Δ/Δ mice were exposed to normoxic or OGD conditions for 6 h in the presence of either pre-clustered EphB2/Fc or anti-IgG Fc. Gene expression was determined by quantitative real-time RT-PCR (mean ± SD; n = 6/4 (N), n = 3/4 (OGD); Student’s t-test) and ELISA (mean ± SD; n = 12/9 (N); Student’s t-test). * p < 0.05