Fig. 1

Microgliosis, abnormal iron accumulation, oligodendrocyte/myelin pathology and axonal injury in the LEWzizi CNS. a Immunohistochemical staining for Iba-1 of lumbar spinal cord grey matter (SpC GM) of 4-month-old (4 M) Lewis and LEWzizi rats. Scale bars, 25 μm b Gene expression analysis of microglia-associated genes in lumbar spinal cord homogenates of 4 M rats. Per gene of interest, color-coded z-scores for each biological replicate (n = 5 per rat strain) are shown as well as the level of up- or downregulation and the false discovery rate (FDR)-corrected p-value in LEWzizi compared with wild-type Lewis tissue. c Turnbull Blue (TBB) staining for the detection of non-haem tissue iron. Representative pictures of DAB-enhanced TBB single labelling as well as double stainings of AEC- or DAB-enhanced TBB with cell type-specific markers Iba-1 (microglia) or Olig2 (oligodendrocytes), respectively, derive from coronal CNS sections of 8-month-old (8 M) Lewis and LEWzizi rats. MesEnc, mesencephalon; scale bars, either 1 mm or 25 μm d Gene expression analysis of oligodendrocyte and myelin genes in lumbar spinal cord homogenates of 4 M rats. Per gene of interest, color-coded z-scores for each biological replicate (n = 5 per rat strain) are shown as well as the level of up- or downregulation and the false discovery rate (FDR)-corrected p-value in LEWzizi compared with wild-type Lewis tissue. e Immunohistochemical staining for CNPase of SpC GM cross sections of 4 M Lewis and LEWzizi rats. Scale bars, 250 μm. f Immunohistochemical staining for APP of a SpC GM cross section of an 8 M LEWzizi rat. APP-positive axonal spheroids and endbulbs are indicated by arrows; an oligodendrocyte exhibiting a strong cytoplasmic APP staining is indicated by an asterisk. Scale bar, 25 μm