Fig. 4

SUMOylation of SET inhibits PP2A and promotes tau phosphorylation. a HEK-293 T cells were transfected with pCDNA3.1 (+), Flag-SET-WT or Flag-SET-K68R, His-SUMO-1 and UBC9 plasmids for 48 h. Cells were lysed and probed for total levels of PP2A via western blotting. b Quantification of the blots described in (a) was performed using ImageJ software. c PP2A activity was measured using commercially available assays. **P < 0.01, ***P < 0.001. (n = 3). d Levels of tau phosphorylation at Ser396, Ser404, AT-8 (Ser202, Ser205) in transfected HEK-293tau cells were assessed by western blotting analysis and e quantified using ImageJ. ** P < 0.01 vs. SET-WT transfection control. f Total tau developed with Tau5 were comparable among each group. All data shown represent the mean ± SD of 3 independent experiments