Fig. 2

Cisplatin induces NSC mitochondrial dysfunction. Neuronal stem cells (NSCs) were treated with 1 μM cisplatin for 12 h. Oxygen consumption rates (OCR) were analyzed using a Seahorse XFe 24 Analyzer and normalized to protein content (a). Mean basal, ATP production-related, and maximum respiratory capacity (MRC) normalized to protein content were calculated (b). Results are expressed as means ± SEM of 3 independent experiments. Data were analyzed using Student’s t-test: ** P < 0.01; ***P < 0.001; **** P < 0.0001. NSCs were treated with 1 μM cisplatin for 8 h followed by 17 h in normal medium. Cells were stained with tetramethylrhodamine methyl ester (TMRM) and monitored immediately by live-cell imaging (c) or flow cytometry (d). NSCs treated with carbonilcyanide p-triflouromethoxyphenylhydrazone (FCCP, 10 μM for 15 min) were used as a positive control. Bar graphs represent mean ± SEM of 3 independent experiments. Data are normalized to mean fluorescence intensity of vehicle-treated cells in each experiment. Data were analyzed using One-way ANOVA followed by Bonferroni’s post-hoc test. *** P ≤ 0.001