Fig. 3

The impact of GJA1 deficiency on AD relevant gene ontologies and AD risk networks. a. Venn diagram for genes overlapping among various differentially expressed genes (DEG) signatures. AST(dn) and AST(up) were down- and up- regulated DEG signatures in Gja1−/− vs. wildtype astrocyte cultures, while AST + NEU(dn) and AST + NEU(up) were down- and up- regulated DEG signatures in Gja1−/− vs. wildtype astrocytes co-cultured with neurons. Top gene ontology for each individual DEGs were shown above each cluster. b. Heatmap of Gene enrichment significance (−log10(p)) for the DEG signatures. Top 10 enriched gene ontologies were shown for each individual DEGs. c. GJA1−/− signatures including AST(all), AST(up), AST(dn), AST + NEU(all), AST + NEU(up) and AST + NEU(dn) are enriched for three AD genetic gene sets including the one identified by the International Genomics of Alzheimer’s Project (IGAP) [1], the Abeta (Aβ) genetic network [16], the AD risk gene list (ADGWAS) [43]