Fig. 5
Differentially regulated pathways and gene ontologies were unaffected by microglial depletion and repopulation. Adult (6–8 weeks old) and aged (16–18 months old) male BALB/c mice were provided diets formulated with vehicle or CSF1R antagonist (PLX5622) for 21 d. After 21 d, all mice were provided vehicle diet for an additional 21 d to allow for repopulation of microglia. After 21 d of repopulation, Percoll-enriched microglia were sorted, and RNA was collected and sequenced. Lists of differentially expressed genes were generated by comparing Aged Control and Aged Repop to Adult Controls (baseMean > 10, Padj < 0.05, and absolute fold change > 1.5) and used for pathway analyses. a Heat map of results from IPA Comparison Analysis of Canonical Pathways, Diseases & Functions, and Upstream Regulators performed on genes differentially expressed in the Aged Control and Aged Repopulation groups compared to Adult Control. All pathways shown were significantly regulated by age (P < 0.05). b Proportions of genes categorized for each GO Biological Process and c GO Molecular Function determined by PANTHER Gene Ontology assessment (n = 6 mice / group)