Fig. 5

Immunohistochemistry for H3K27me3 in PFA and PFB tumors. All PFA tumors demonstrated reduced H3K27me3 expression (80% or less). Approximately 38% of them showed reactivity in less than 5% of tumor cells (a, e), and the remaining cases showed labeling in 5–50% of tumor cells (b, e). In contrast, most PFB tumors retained intact H3K27me3 expression (> 80% labeled nuclei) (c, e). A few PFB tumors, however, showed labeling in 10–60% of cells, which were categorized as reduced expression of H3K27me3 (d, e). e, a histogram of the percentage of labeled nuclei in PFA and PFB tumors. f, a confusion matrix for actual and predicted subgroup by H3K27me3 immunohistochemistry when PFB was defined as intact H3K27me3 expression and PFA as reduced expression