Fig. 3

Changes in GFAP protein levels in ephrinB1-Fc treated Müller cells and ephrinB1-Fc injected retinas. a, Representative immunoblots showing the changes in GFAP protein levels in Müller cell extracts treated with IgG-Fc (500 ng/ml) (Ctr) and those treated with ephrinB1-Fc (500 ng/ml) for different periods of time (1–24 h). b, Bar charts summarizing the average densitometric quantification of immunoreactive bands of GFAP under the conditions as shown in A. n = 6 for all groups. c, Immunofluorescence labeling showing GFAP expression in rat retinal vertical slices taken from normal saline-injected retina (c1) (Ctr) and ephrinB1-Fc-injected retinas on 1 and 2 weeks (c2 and c3) after the injection. Scale bar, 20 μm, for all the images. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer