Fig. 2

EphrinB/EphB forward signaling is activated in retinal Müller cells by IOP elevation and ephrinB1-Fc treatment. a, Representative immunoblots showing the changes in EphB1 and phosphorylated EphB (p-EphB) expression in sham-operated (control, Ctr) and COH retinal extracts from G1w to G4w. b-d, Bar charts summarizing the average densitometric quantification of immunoreactive bands of p-EphB (b), EphB1 (c) and the average p-EphB/EphB1 ratios (d) obtained in COH retinal extracts from G1w to G4w. n = 5 for all groups. e, Representative immunoblots showing the changes in EphB1 and p-EphB levels in Müller cell extracts treated with IgG-Fc (500 ng/ml) (Ctr) and those treated with ephrinB1-Fc (500 ng/ml) for different periods of time (1–24 h). f-h, Bar charts summarizing the average densitometric quantification of immunoreactive bands of p-EphB (f), EphB1 (g) and the average p-EphB/EphB1 ratios (h) in Müller cells treated with ephrinB1-Fc for different periods of time, as compared to those in control condition. n = 5 for all groups. i, Representative immunoblots showing the changes in EphB1 and p-EphB expression in normal saline-injected retina (Ctr), and ephrinB1-Fc-injected retinas (0.5 μg/μl, 2 μl) at different post-injection times. j-l, Bar charts summarizing the average densitometric quantification of immunoreactive bands of p-EphB (j), EphB1 (k) and the average ratios of p-EphB/EphB1 (l) in retinal extracts under control condition and those of ephrinB1-Fc-injected retinas at different post-injection times (3 d and 7 d). n = 4 for all groups. All the data are normalized to their corresponding β-actin and then to Ctr. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs. Ctr