Fig. 3

OHC loss in the basal region of the cochlea in NPC1-KO mice. a. Cytocochleograms of WT and NPC1-KO littermates from a heterozygous breeder pair, where the % OHC survival is plotted along the length of cochlea. Vertical black dotted lines indicate the regions corresponding to 12 and 27 kHz. b-c Immunofluorescent images showing the basal region (~ 75% of the distance from the apex, as indicated by the pale blue arrow in (a) for a P56 female WT (b) and a P49 male NPC1-KO (c) In this region, scattered OHC loss is observed in NPC1-KO but not in WT. Whole-mount OC sections were stained with anti-N-mprestin antibody (green) and phalloidin-Alexa 546 (red). Scale bars, 100 μm