Fig. 2

Serial transmission and characterization of the strain produced upon inoculation of rPrPres^{PE + PolyA}. a Western blots of brain materials from animals inoculated with PMCA-derived rPrPres^{PE + PolyA} and sacrificed at 622 days post-inoculation. One and ten percent brain homogenates from SSLOW-inoculated animals are provided as a reference. b Comparison of amplification efficiency of PrP^Sc in PMCA and dsPMCA that utilizes desialylated substrate. PMCA or dsPMCA reactions were seeded with SSLOW^{PE + PolyA}, SSLOW or 263K brain materials serially diluted to up to 10¹⁰-fold as indicated, subjected to one amplification round and analyzed by Western blot. In a manner similar to SSLOW, amplification efficiency of SSLOW^{PE + PolyA} increased drastically in dsPMCA conditions relative to that of PMCA conditions. c Western blots of brain materials of the first and second passages of SSLOW^{PE + PolyA}. Arrows indicate animals that were used for the serial transmission. 3F4 antibody was used. d Kaplan-Meier survival curves for animals inoculated with SSLOW (circles) or SSLOW^{PE + PolyA} (2nd passage, two individual groups, squares and triangles)