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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Preserving prion strain identity upon replication of prions in vitro using recombinant prion protein

Fig. 2

Serial transmission and characterization of the strain produced upon inoculation of rPrPresPE + PolyA. a Western blots of brain materials from animals inoculated with PMCA-derived rPrPresPE + PolyA and sacrificed at 622 days post-inoculation. One and ten percent brain homogenates from SSLOW-inoculated animals are provided as a reference. b Comparison of amplification efficiency of PrPSc in PMCA and dsPMCA that utilizes desialylated substrate. PMCA or dsPMCA reactions were seeded with SSLOWPE + PolyA, SSLOW or 263K brain materials serially diluted to up to 1010-fold as indicated, subjected to one amplification round and analyzed by Western blot. In a manner similar to SSLOW, amplification efficiency of SSLOWPE + PolyA increased drastically in dsPMCA conditions relative to that of PMCA conditions. c Western blots of brain materials of the first and second passages of SSLOWPE + PolyA. Arrows indicate animals that were used for the serial transmission. 3F4 antibody was used. d Kaplan-Meier survival curves for animals inoculated with SSLOW (circles) or SSLOWPE + PolyA (2nd passage, two individual groups, squares and triangles)

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