Fig. 5
BoNT/B injection into the contralateral side of the seeds injection reduced the propagation of a-syn in the connected areas. Top: Schematic view of the injection sites and the experimental procedures. Injection with BoNT/B to the contralateral side of the seeds injection was conducted 3 days before or 1 day after injection with mouse a-syn PFFs. As a control, physiological saline was administered instead of BoNT/B 3 days before or 1 day after injection of mouse a-syn PFFs. The mice were dissected 1.5 months later. (a, b) Injection with saline or BoNT/B 3 days before injection with mouse a-syn PFFs . a: Threads (Str p = 0.0468, Amyg p = 0.0025) b: Cytoplasmic inclusions (Str p = 0.0002, Amyg p = 0.0002, EC p = 0.0101). (c, d) Injection with saline or BoNT/B 1 day after injection with mouse a-syn PFFs. (a-d) The total area of p-syn-positive inclusions (deposits) was quantified for each region (Str, CTX, EC, Amyg) of the brain. Ips: mouse a-syn PFFs injection side, contra: the contralateral side to the mouse a-syn PFFs injection side. Horizontal axis: Brain region; Vertical axis: Total area of p-syn-positive inclusions (μm2)/unit area (mm2). The p-syn-positive were divided into neuritic inclusions (threads) and cytoplasmic inclusions, and quantified separately. n = 5 mice per group, unpaired t-test with Student’s correction and Bonferroni correction (significance level 0.05/2 = 0.025); Data are the mean area per region ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001. Str: striatum, CTX: cortex, Amyg: amygdala, EC: entorhinal cortex