Fig. 3
The propagation of a-syn deposits in the contralateral hemisphere was reduced after callosotomy. Callosotomy was conducted 1 day before or 1 day after injection with mouse a-syn PFFs. As a control, a-syn PFFs were injected into mice without callosotomy. (a-c)Schematic representation of the experimental protocol and p-syn deposits detected with anti-p-syn#64. Scale bars, 50 μm. (a, d) Injection with a-syn PFFs only (no callosotomy) (Str p = 0.0171). (b, e) Callosotomy 1 day before injection with a-syn PFFs (Str p = 0.0098, CTX p = 0.0205, EC p = 0.0025, Amyg p = 0.0476). (c, f) Callosotomy 1 day after injection with a-syn PFFs. (d-f) The total area of p-syn-positive inclusions (deposits) was quantified for each region (Str, CTX, EC, Amyg) of the brain. Horizontal axis: Brain region; Vertical axis: Total area of p-syn-positive inclusions (μm2) per unit area (mm2). Data is represented as mean area per region ± SEM, n = 5 mice per group, paired t-test; *p < 0.05; **p < 0.01. Str: striatum, CTX: cortex, EC: entorhinal cortex, Amyg: amygdala