Fig. 2
P-syn inclusions were found in neural systems directly or indirectly connected with the striatum where a-syn seeds were injected. a Distribution of p-syn pathology in a-syn PFFs-injected mouse brain 6 months after injection. Red dots indicate neuritic inclusions (threads) or cytoplasmic inclusions. Blue circles indicate the injection site in the striatum. L = left hemisphere of the brain; R = right hemisphere. (b-f) Total area of the p-syn-positive inclusions (deposits) quantified chronologically at 0.75, 1.5, 3, and 6 months for each region (Str, CTX, SN, EC, and Amyg) of the brain. Horizontal axis: Time following a-syn PFFs injection; vertical axis: Total area of p-syn-positive inclusions (μm2) per unit area (mm2). b Str (0.75 month p = 0.0062, 1.5 month p = 0.031, 3 month p = 0.0413, 6 month p = 0.0117), c CTX, d SN (1.5 month p = 0.0122, 6 month p = 0.0013), e EC (6 month p = 0.0235), and f Amyg 3 month p = 0.0025, 6 month p = 0.0189. Mixed effect model with contrast-based testing: Fig. 2b p < 0.0001, Fig. 2c p < 0.0001, Fig. 2d p < 0.0001, Fig. 2e p < 0.0001, Fig. 2f p < 0.0001. Data is represented as mean area per region ± SEM, n = 5 mice per group. Paired t-test and the mixed effect model with contrast-based test was carried out to evaluate the significance of trends in time related response using the linear contrast; *p < 0.05, **p < 0.01. Str, striatum; CTX, cortex; SN, substantia nigra; EC, entorhinal cortex; Amyg, amygdala