Fig. 4

C9orf72 co-localizes with synaptic vesicles in human iPSC derived motor neurons. a C9orf72 positive puncta (green) are seen in the axons of 30 day old human iPSC derived motor neurons which consistently co-localize with SMCR8 (red, upper panel) and partially co-localize with LAMP2 as lysosomal marker (red, middle panel) or with the synaptic vesicle marker synaptophysin (red, lower panel). Nuclei are stained with DAPI (blue) in the merged images. C9orf72 labeled with 12E7 antibody in the upper panel and with 1C1 in the middle and lower panel. b Graph showing the percentage of C9orf72 positive puncta co-localizing with SMCR8, LAMP2 or synaptophysin. Values are shown as mean ± SD