Fig. 7

Ppt1 deficient (Ppt1^−/−) neurite outgrowth is impaired. Wild type (WT) and Ppt1^−/− neuronal cultures were stained with MAP2 to visualise neuronal processes and DAPI to label all nuclei. Neurite length was measured using ImageJ. a Mean neurite length is significantly shorter in Ppt1^−/− neuronal cultures than in WT cultures after 7, 9 and 14 days in vitro (DIV). b The length of the longest neurites, assumed to be the axon is significantly reduced in Ppt1^−/−neurons compared to WT neurons after 7, 9 and 14 DIV. c Ppt1^−/− neurons stained for Map2 appear to have shorter neurites than their WT counterparts and appear less complex. d The number of primary neurites did not differ between WT and Ppt1^−/− neuronal cultures after 7 days in vitro (DIV). e Ppt1^−/− neurons had significantly fewer secondary neurites than their WT counterparts after 7 DIV. f Significantly fewer tertiary neurites were present in Ppt1^−/−neuronal cultures. (Data shown as Mean ± SEM using a one way ANOVA, n = 3). Scale bar = 50 μm