Fig. 2

Expression of SCF, VEGF and IL-4 was significantly higher in Ppt1 deficient (Ppt1^−/−) astrocytes after stimulation. Supernatant was collected from wild type (WT) and Ppt1^−/− astrocyte cultures kept under basal and stimulated conditions for 24 h. Cytokine release was assessed using an ELISA kit (Signosis) and calculating changes in expression between basal and stimulated conditions. No statistically significant changes were observed in the secretion of Epidermal growth factor (EGF), Interferon γ (IFNγ), Tumour necrosis factor α (TNFα), Expression of Basic flbroblast growth factor (FGFb) and Interleukin 1-α (IL1α). Stem cell factor (SCF), Vascular endothelial growth factor (VEGF) and Interleukin-4 (IL-4) was significantly higher in Ppt1^−/− astrocyte cultures following stimulation than in WT cultures. (Data shown as Mean ± SEM using a t-test, n = 3)