Fig. 2

RNA binding proteins change in their association to tau in tauopathy. Using Tau13 antibody, tau was immunoprecipitated from the cortex of either 2.5 month old induced or uninduced rTg4510 brains.The co-immunoprecipitating proteins were subsequently identified by mass spectrometry. A variety of nucleotide binding and ribonucleoproteins show differential association to tau in rTg4510 tissue compared to WT tissue as indicated by significantly upregulated and downregulated annotation terms (a). Part (b) represents a network diagram of proteins enriched in the tau interactome. Red nodes denote proteins whose association with tau is ≥5-fold increased in Tg4510 vs uninduced control. Blue nodes denote proteins whose association with tau is ≥5-fold decreased in Tg4510 vs control. Node size is proportional to degree of replication (N = 4), non-replicating proteins excluded. Key proteins include EWSR1, HNRNPA0, PABP, PCBP2, DDX5, TAF15, FMR1, RPL and RPS family members, and eIF4a2