Fig. 4

E90 vaccination of dams protects neonates from ZIKV-induced microcephaly. a. Comparison of body weights and brain sizes of mock–infected and ZIKV-infected vaccinated and unvaccinated groups as shown in Fig. 1d. Neonatal brains were injected with ZIKV or medium at P1 and inspected at P10. Lower panels: measurements of body and brain weights. For body weight, Mock + Veh: n = 15, ZIKV + Veh: n = 15, ZIKV + E90: n = 10. For brain weight, Mock + Veh: n = 5, ZIKV + Veh: n = 5, ZIKV + E90: n = 4. b. Nissl staining of coronal sections. Right panel: thickness of the cortical layer. Mock + Veh: n = 12/4, ZIKV + Veh: n = 8/3, ZIKV + E90: n = 11/4. All data are means ± SEM. Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. ns: not significant. n: # of slices/# of individual brains. Scale bar = 1 cm (a), 1 mm (b)