Fig. 2

Parabiosis shows that the mononuclear cell response within a retina to an ONC is not mediated by recruited, circulating macrophages. a Experimental design of the parabiotic mice and controls. Retina groups and treatment are indicated. For non-injured mice (no ONC; groups 1, 2, and 4), both retinas from individual mice constituted individual samples. For ONC mice (groups 3 and 5), only the retinas from injured eyes (black filled) constituted samples. b Flow cytometry analysis of blood showing successful parabiosis. Circulating monocytes (CD45⁺CD11b⁺) were analyzed four to 6 months post-parabiosis for GFP. Results presented as GFP^lo or GFP^hi monocytes as a percent of all blood mononuclear cells. For parabiotic mice, both members of a pair were analyzed for GFP^lo and GFP^hi monocytes. c Flow cytometry analysis of retinal myeloid cells. Non-parabiotic mice were aged-matched to parabiotic mice. Mice were analyzed 7–9 days post-ONC. Results presented as number of GFP^lo or GFP^hi myeloid cells (CD45^medCD11b⁺Ly6G⁻) per retina. Statistics were done by ANOVA with Tukey HSD post-test with number of mice analyzed indicated, * = p value of < 0.05, ns = not significant