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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Rac1 activation links tau hyperphosphorylation and Aβ dysmetabolism in Alzheimer’s disease

Fig. 4

The nuclear transporter inhibitor LMB blocks Rac1-induced translocation of SET. SH-SY5Y cells at 10 days of RA differentiation were treated with Rac1-WT and Rac1-L61F37A, with or without LMB in order to block Rac1-peptide mediated SET translocation. After 48 h, cells were fixed and stained against pT181 tau epitope (green), SET (magenta), F-actin (red), and DAPI (blue) was used to visualize nuclei. Representative images show that, in control condition SET expression is restricted to the nucleus. After Rac1-WT and Rac1-L61F37A treatments, SET presence is observed also outside cell nuclei, whereas SET translocation doesn’t occur when LMB is added together with Rac1 mutant treatments. In the same way, tau phosphorylation at the epitope pT181 is decreased in presence of LMB. Scale bar 25 μm

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