Fig. 3

Rac1 mutant peptides induce tau hyperphosphorylation mediated by SET translocation. a Primary cortical neurons treated between DIV11 and DIV14 with Rac1 mutant peptides. After 24 h, cells were fixed and stained to visualize SET, dendrites (MAP2), and nuclei (DAPI). These representative images were obtained from one of three independent experiments. Scale bar 10 μm. b-c Representative blots and densitometry of subcellular fractionation indicating the levels of SET in the membrane (SET/GluR1) and nuclear fractions (SET/LaminB) in the same conditions as in A. 15 μg of protein lysate were loaded for the nuclear fraction. Due to the low yield, the membrane fraction was not quantified. d-e Representative blots and corresponding quantification of tau pT181 phosphorylation and Tau-5 normalized against GAPDH levels, and pT181/Tau5. ~ 7 μg of protein lysate were loaded