Tau localises to the nucleolus in undifferentiated and differentiated Neuroblastoma (SHSY5Y) cells. a Immunogold labelling of undifferentiated cells with T-Tau antibody using 10 nm gold particle-conjugated secondary antibody showed tau gold particles within the Nucleolus (1). Nucleus is highlighted in pink and nucleolus used in second panel is highlighted in blue. The region 1, is identified by a black box. Representative immunofluorescence fluorescence images showing colabelling for nP-Tau and FBL in undifferentiated (b) and differentiated (c) cells using Tau 1 antibody shows clear punctate distribution of nP-Tau colocalised with fibrillarin (FBL).