Phosphorylation of different tau sites during progression of Alzheimer’s disease

Table 2 List of primary antibodies for histology

Antigen	Clone	Species	Source	Order #	Dilution	Incubation time
Total human tau	HT7	mouse	Pierce Biotechnology, USA	MN1000	1:500	overnight at 4 °C
pThr231	AT180	mouse	Pierce Biotechnology, USA	MN1040	1:200	overnight at 4 °C
pSer202 + pThr205	AT8	mouse	Thermo Scientific, USA	MN1020	1:200	overnight at 4 °C
pSer199	polyclonal	rabbit	Invitrogen Corporation, USA	44-734G	1:500	1 h at RT
pTyr18	9G3	mouse	MédiMabs, Canada	MM-0194-P	1:1000	1 h at RT
pSer396	PHF13	mouse	Cell Signaling Technology, USA	9632	1:500	1 h at RT
pSer262	polyclonal	rabbit	MBL International, USA	AT-5020	1:250	overnight at 4 °C
pSer422	5.6.11	mouse	Roche Diagnostic, Switzerland	N/A	1:800	overnight at 4 °C

ISSN: 2051-5960