Fig. 3

DIPG- and aGBM-associated macrophages exhibit distinct gene expression profiles (a) Principal components analysis of the top 500 varying genes across all samples demonstrates clusters corresponding to normal cortical microglia (green), DIPG-associated macrophages (blue), and aGBM-associated macrophages (red). b-c Volcano plot of log-fold change against adjusted p value for genes between normal cortical microglia and DIPG-associated macrophages (b), and between DIPG-associated macrophages and aGBM-associated macrophages (c). Red dots represent adjusted p value < 0.05, and selected significantly differentially expressed genes are identified (d) Heat map of normalized count values for differentially expressed genes between DIPG- and aGBM-associated macrophages. Hierarchical clustering demonstrates a distinct difference between the two groups