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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: A common antigenic motif recognized by naturally occurring human VH5–51/VL4–1 anti-tau antibodies with distinct functionalities

Fig. 3

Detection of immunoreactivity in human brain tissue by CBTAU-27.1 and CBTAU-28.1 and affinity-improved variants. Immunohistochemistry was performed on 5 μm thick formalin-fixed paraffin embedded sections of the hippocampal region using a 0.1 μg/ml antibody concentration. Immunodetection using CBTAU-27.1 (a-d), dmCBTAU-27.1 (e-h), CBTAU-28.1 (i-l), dmCBTAU-28.1 (m-p) and PHF-tau-specific mouse antibody AT8 (q-t) in control and AD brain tissue without or with heat pretreatment using sodium citrate buffer. Gallyas staining for detection of NFTs and neuropil threads is shown from the same control and AD case of corresponding areas for comparison (u, v). Immunoreactivity was visualized using DAB (brown) and nuclei were counterstained with haematoxylin (blue). Representative areas of the CA1/subiculum of the hippocampus are shown. Scale bars represent 50 μm

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