Fig. 4

Formation of insoluble protein aggregates in A53T-aSYN overexpressing animals. a Staining for p62 (red, upper row) and Ubi-1 (red, lower row) revealed abundant p62- and Ubi-1-positive aggregates in the LC-region. These aggregates were found in close proximity to, but did not co-localize with TH-positive (green) LC cells, which were positive for p-aSYN (magenta). b Representative images of p62- (upper left) and Ubi-1 (lower left) positive aggregates in A53T-aSYN animals. No aggregates were observed in Luc overexpressing animals at any time point (right column). c p62-, Ubi-1 and aSYN stainings after proteinase K (PK) digestion or without digestion (Ø PK, lower right) confirmed that p62- (upper left), Ubi-1- (upper right) and human aSYN-positive (lower left) aggregates were insoluble. Scale bars 50 μm in a and c, 25 μm in b