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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies

Fig. 3

Primary cortical neurons expressed active TAOK-pS181. a Neurons were cultured for 3, 7, 10 or 14 DIV and lysates immunoblotted with antibodies to detect TAOK-pS181, TAOK1, TAOK2, tau-pS202/T205/S208 (AT8), tau-pS262/S356 (12E8), total tau or BIII tubulin as indicated. b Neurons were transfected with pCAGS-Myc-TAOK1-IRES-EGFP (TAOK1-GFP), pCAGS-Myc-TAOK2-IRES-EGFP (TAOK2-EGFP), pCAGS-Myc-TAOK2–1-349-IRES-EGFP (TAOK2–1-349-EGFP), pCAGS-Myc-TAOK2-K57A-IRES-EGFP (TAOK2 K57A-EGFP) or pCAGS-IRES-EGFP (EGFP) plasmids, as indicated. 7 DIV transfected neurons were fixed and immunostained with antibodies to detect active TAOK-pS181 (red) and Myc (magenta). Nuclei were counterstained with DAPI (blue) and representative maximal projection of confocal z-stack images is shown. Scale bar = 10 μm

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