Fig. 5
D409H GBA1 expression leads to the accumulation of pathologic α-synuclein aggregates in the brainstem and SNpc of A53T α-synuclein transgenic mice. a, Representative images of p-α-Syn positive signals in the brainstem . Immunohistochemistry with phosphor-S129 α-synuclein (p-α-Syn) antibody was conducted in the lateral vestibular nucleus of the brainstem of the indicated genotypes at 6 months of age. The scale bar represents 50 μm. b, Representative images of p-α-Syn signals in the SNpc of the indicated genotypes at 6 months of age at low-magnification and high-magnification. The scale bar is 200 μm. c, Semi-quantitative grading of p-α-Syn pathology in the indicated genotypes. d and e, Dot-blot analysis of brainstem (BS) and ventral midbrain (VMB) of bigenic mice (n = 3) with α-synuclein filament antibody was performed and quantified. f and g, Accumulation of high molecular weight species of α-synuclein in the ventral midbrain was assessed in the VMB. The ventral midbrain lysates were immunoblotted with α-Syn antibody. The high molecular weight species were quantified