Fig. 3

MAC inhibitor completely inhibits IL-1β synthesis while, in contrast, C5aR1 inhibition only partially decreases expression. Immunostaining for interleukin 1 beta (IL-1β)/pro-IL-1β, indicator of NLRP3 inflammasome activation, shows reactivity within the spinal cord of no drug mice (n = 4, arrows in a), on cells with a glial morphology (a, b). IL-1β/pro-IL-1β was never detected in the cords of C6 antisense-treated mice (n = 4) (c), while PMX205-treated mice showed sparse reactivity (n = 3) (arrows in d, e). Tissue was collected at relapse. Scale bars (a, c, d) 25 μm, (b, e) 5 μm. Hematoxylin was used as counterstain in (a-e)