Fig. 8
From: Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods
Effects of inactivation treatments of synthetic α-syn fibrils on seeding activity in SH-SY5Y cells. Serial 10-fold dilutions of human α-syn fibrils treated with 1% SDS (red), boiling (green), autoclaving (AC) at 134 °C (orange) or autoclaving at 134 °C with 1% SDS (purple) were introduced into SH-SY5Y cells transiently expressing human WT α-syn. Phosphorylated α-syn accumulated in SH-SY5Y cells exposed to serial dilutions of synthetic human α-syn fibrils were quantified after each treatment. Band intensities from the immunoblot analyses shown in Additional file 6: Figure S4 were measured. The results are expressed as means ± SEM (n = 3)