Fig. 2
From: Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods
Characterization of α-syn aggregates extracted from brains of synucleinopathy patients. a Immunoelectron microscopy of sarkosyl-insoluble fractions extracted from DLB (left) and MSA (right) patients’ brains. Electron micrographs show fibrous structures positive for PSer129 antibody EP1536Y, that were labeled with secondary antibody conjugated to 5 nm gold particles. Scale bar, 50 nm. b Immunoblot analyses of sarkosyl-insoluble fractions prepared from brains of synucleinopathy patients. Sarkosyl-insoluble α-syn (22, 29 and 37 kDa in DLB, and 22 and 32 kDa in MSA) were detected by LB509 (left), anti-syn 131–140 (center) and Syn 102 (right) antibodies