Fig. 4
From: Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer’s disease brain
SphK2 protein expression is increased in nuclear compartments in AD group. Subcellular localization of both full length and cleaved SphK2 was analyzed by western blot. a Representative western blot showing SphK2 expression in cytoplasmic and in nuclear fraction of control (n = 5) or AD groups (n = 10) of tissue samples from the frontal cortex (a1) or the hippocampal area (a2). Relative expression of full length SphK2 (b) and cleaved SphK2 (c) proteins in cytoplasmic or nuclear fractions was analyzed in the frontal cortex (b1-c1) and the hippocampal area (b2-c2) of tissue extracts in AD group as compared to control group. After normalization (using stain-free imaging technology), data were expressed as percentage of SphK2 expression levels in AD versus control group. Statistical significance was determined using one-way ANOVA test. Columns, mean; bars, SEM. **p < 0.01, ***p < 0.001 vs control group