Fig. 3

Correlation between the proportion of cleaved SphK2 and Aβ*27 oligomers in frontal cortex, hippocampal area and cerebellum. a Representative blot showing Aβ oligomers (upper panel) and SphK2 (lower panel) full length and cleaved proteins in frontal cortex (a1), hippocampal area (entorhinal cortex and CA1) (a2) and cerebellum (a3) tissue samples. Aβ oligomers and SphK2 expression were normalized using stain-free imaging technology. The relative abundance of total Aβ oligomers (b) and Aβ*27 oligomers (c) was analyzed in the three structures. d Cleaved SphK2 expression, in control (n = 6) and AD (n = 10) groups in frontal cortex, hippocampal area and cerebellum (control n = 3 and AD n = 5). Statistical significance was determined using one-way ANOVA. Columns, mean; bars, SEM. *p < 0.05, **p < 0.01, # p < 0.08 vs control group. e Scatter plots depicting the relationship between Aβ*27 oligomers (x axis) and cleaved SphK2 expression (y axis) in frontal cortex (e1), hippocampal area (e2) and cerebellum (c3). Regression lines are displayed