Fig. 7
Prophylactic treatment with BLZ945 1 week before and during 5-week cuprizone intoxication revealed reduced oligodendrocyte numbers, appearance of myelin debris and axonal pathology in the external capsule but not in the corpus callosum. a Representative images from immunohistological stainings in the external capsule and corpus callosum detecting debris of myelin basic protein (dMBP). Myelin debris were obvious in the external capsule with BLZ945 and cuprizone treatment while no obvious change could be observed in the corpus callosum. b Corresponding quantitative analysis of the immunohistochemistry for dMBP-positive area in the corpus callosum and external capsule. Please note: The threshold for the staining of the control groups were below the image analysis parameters used. Values were normalized to those of control vehicle mice. c Representative images from immunohistological stainings in the external capsule and corpus callosum detecting neurofilament (SMI312) for the different treatment groups at week 5. Loss of axonal neurofilaments was obvious in the external capsule with BLZ945 and cuprizone treatment while no change could be observed in the corpus callosum. d Corresponding quantitative analysis of the immunohistochemistry for SMI312-positive area in the corpus callosum and external capsule. e Representative images from immunohistological stainings in the external capsule and corpus callosum detecting mature oligodendrocytes (GST-π) for the different treatment groups at week 5. Reduced oligodendrocyte numbers were observed in the external capsule while an increase in the corpus callosum was obvious with BLZ945 and cuprizone treatment. f Corresponding quantitative analysis of the immunohistochemistry for GST-π-positive soma numbers in the corpus callosum and external capsule. Values were normalized to those of control vehicle mice. Group sizes: for all treatment groups n = 4–5. Data are shown as means±SEM. Scale bars: 100 μm. Statistics: Turkey’s multiple comparison test one-way ANOVA **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, n.s.: not significant), cpz: cuprizone, cc: corpus callosum, ec: external capsule