Fig. 5

Prophylactic treatment with BLZ945 1 week before and during 5-week cuprizone intoxication inhibited demyelination in the corpus callosum but not in the external capsule. a Schematic diagram of the experimental setup for the prophylactic treatment. Groups consisted of mice pretreated for 1 week with vehicle or 169 mg/kg BLZ945 (p.o., qd) on normal food. Control food was continued or then switched to 0.2% cuprizone with concomitant continuation of vehicle or 169 mg/kg BLZ945 (p.o., qd) treatment for 5 weeks. MRI measurements were performed at week 0 (baseline) as well as week 3 and week 5 during cuprizone intoxification. Mice were killed at week 5 immediately after the last MRI measurement. b Representative MRI brain images for mice treated with either BLZ945 (p.o., qd, 169 mg/kg) or vehicle before (1 week) and during 0.2% cuprizone intoxification for 5 weeks. red arrows: corpus callosum, green arrows: external capsule. c Representative MRI brain images indicating the analyzed brain regions (red: corpus callosum, green: external capsule). d, e Quantification of the MRI contrast and MTR in the corpus callosum for the different treatment groups showing reduced MRI contrast and enhanced MTR for the corpus callosum compared to the external capsule. For all groups: n = 5. Data is shown as mean ± SEM. Statistics: Turkey’s multiple comparison test one-way ANOVA (*: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001), n.s.: not significant, a.u.: arbitrary units, MRI: magnetic resonance imaging, MTR: magnetization transfer ratio