Table 2 Antibodies and immunohistochemistry conditions
From: Pyroglutamate and Isoaspartate modified Amyloid-Beta in ageing and Alzheimer’s disease
Clone | Specificity | Company | Modified amino acid | Antigen Abbreviation | Dilution | Antigen retrieval method |
|---|---|---|---|---|---|---|
22C8 | IsoAspartate Aβ | Elan Pharmaceuticals | Aspartate in position 1,7 | IsoD-Aβ | 1:10,000 | Formic acid 80% for 30 min |
337.48 | pyroglutamate Aβ | BioLegend | Glutamate in position 3 | pE3-Aβ | 1:100 | Formic acid 100% for 3 min + EDTA buffer and microwave |
4G8 | Aβ and APP | BioLegend | n/a | AβPP | 1:2000 | Formic acid 80% for 30 min |
82E1 | N-terminus end specific | IBL | n/a | Aβ | 1:100 | Formic acid 80% for 30 min |
AT8 | Phosphorylated tau | ThermoFisher Scientific | Ser202 and Thr205 | p-TAU | 1:500 | Sodium citrate buffer and pressure cooker |