Fig. 3
Analysis of PrPSc PK-resistance (a, c) and thermo-solubility (b, d). Representative WBs of case #3 (upper) and a np-CJDMM1 case (lower) are shown in a and b. Membranes were incubated with the primary antibody 3F4. Relative molecular masses are expressed in kDa. c PK digestion profiles. ED50 represents the PK concentration needed to digest 50% of PrPSc (expressed as mean ± standard deviation). d Thermo-solubilization profiles. T50 represents the temperature needed to solubilize 50% of PrPSc (expressed as mean ± standard deviation). No statistically significant differences were observed in both ED50 and T50 between p- and np-CJDMM1 (ED50, Mann-Whitney Rank Sum Test, P = 0.570; T50, t-test, P = 0.306)