Fig. 6

Cln3 ^−/− astrocytes show differences in their ability to secrete proteins. Secreted protein levels were quantified from supernatants collected from wild type (WT) and Cln3-deficient (Cln3 ^−/−) astrocyte cultures grown under basal conditions or after stimulation with LPS/IFNγ. Examples of chemokines (a), neuroprotective factors (b) and proteins secreted at elevated levels by Cln3 ^−/− astrocytes following activation (c), are shown. a Cln3 ^−/− astrocytes secreted significantly less MIP-1ß, MIP-1α and MCP-1 when treated with LPS/IFNγ than did WT astrocytes. There was no significant difference between untreated WT and Cln3 ^−/− samples. b Cln3 ^−/− astrocytes secreted significantly less IL-6, TNF-α and VEGF upon activation than did WT astrocytes. There was no significant difference between untreated samples. c Cln3 ^−/− astrocytes secreted significantly more CRP and IgA after 24 h exposure to LPS/IFNγ, no such change was observed in WT astrocytes