Fig. 4

Cln3 ^−/− astrocytes have a disrupted cytoskeleton. The cytoskeletal organization of wild type (WT) and Cln3-deficient (Cln3 ^−/−) astrocytes was determined by immunostaining with GFAP (red in a, b) to visualize intermediate filaments, phalloidin to visualize F-actin (red in c, d), and α- or β-tubulin to visualize microtubules (green in e, f and in g, h, respectively). DAPI was used to visualize nuclei (blue). WT astrocytes had a well-organized intermediate filament, F-actin and microtubule cytoskeleton (arrowheads in a, c, e, g), while Cln3 ^−/− astrocytes had a highly disrupted intermediate filament and F-actin cytoskeleton (arrowheads in b and d) but their microtubule structure appeared normal (arrowheads in f and h). The cell body size of Cln3 ^−/− astrocytes appeared larger than that of WT astrocytes. Scale bar = 25 μm