Fig. 9

The amount of MC1 tau conformers and pSyk (Y525/526) levels cross-influence each other. Sections from Tg Tau P301S (n = 4, 47 ± 3.1-week-old) were stained with antibodies against tau conformers (MC1, purple) and pSyk (Y525/526, green). Fluorescent intensities of MC1 and pSyk were measured using Zen Blue 2.1 (Zeiss). Three different neuronal fractions were differentiated: a neurons singly immunopositive for MC1, (b) neurons singly immunopositive for pSyk and (c) neurons immunopositive for both MC1 and pSyk (colocalized). d Fluorescent intensities of MC1 were compared between neurons singly MC1 positive (purple) and neurons exhibiting a colocalization of MC1 and pSyk (purple-green-striped). Two-tailed Mann–Whitney test revealed a significant increase (p < 0.05) of MC1 fluorescent intensity in neurons exhibiting a colocalization of MC1 and pSyk compared to neurons singly immunopositive for MC1. e Fluorescent intensities of pSyk were compared between neurons singly immunopositive for pSyk (green) and neurons that show an immunoreactivity for both pSyk and MC1 (purple-green-striped). Two-tailed Mann–Whitney test revealed a significant increase (p < 0.01) of pSyk fluorescent intensity in neurons immunoreactive for both pSyk and MC1 compared neurons that are singly immunopositive for pSyk. The scale bar represents 10 μm. The error bars represent SEM