Fig. 5

iP subunit deficiency alters cytokine secretion profile in adult microglia in vitro. a Schematic representation of experimental procedure for microglia isolation, LPS stimulation and cytokine analyses. b Cytokine levels in supernatant of cultured enriched microglia form 120d old mice at baseline assessed by Meso Scale V-PLEX Plus Pro-inflammatory Panel 1 (mouse) kit (Meso Scale Discovery) (n = 3–4 wells per group, TNFα: LMP7^+/+ vs. APPPS1;LMP7^+/+: p = 0.0275; LMP7^−/− vs. APPPS1;LMP7^+/+: p = 0.0394; APPPS1;LMP7^−/− vs. APPPS1;LMP7^+/+: p = 0.0252; IL-6: LMP7^+/+ vs. APPPS1;LMP7^+/+: p = 0.0095; LMP7^−/− vs. APPPS1;LMP7^+/+: p = 0.0307; APPPS1;LMP7^−/− vs. APPPS1;LMP7^+/+: p = 0.0471; one-way ANOVA followed by Bonferroni post-tests). c Cytokine levels in supernatant of cultured enriched microglia isolated from 120d old mice after 14 h LPS stimulation assessed by Meso Scale V-PLEX Plus Pro-inflammatory Panel 1 (mouse) kit (Meso Scale Discovery) (n = 3–4 wells per group, TNFα: LMP7^+/+ vs. APPPS1;LMP7^+/+: p = 0.0002; LMP7^−/− vs. APPPS1;LMP7^+/+: p = 0.0002; APPPS1;LMP7^−/− vs. APPPS1;LMP7^+/+: p = 0.003; IL-6: LMP7^+/+ vs. APPPS1;LMP7^+/+: p = 0.0002; LMP7^−/− vs. APPPS1;LMP7^+/+: p < 0.0001; APPPS1;LMP7^−/− vs. APPPS1;LMP7^+/+: p < 0.0001; IL-1β: LMP7^+/+ vs. APPPS1;LMP7^+/+: p < 0.0001; LMP7^−/− vs. APPPS1;LMP7^+/+: p < 0.0001; APPPS1;LMP7^−/− vs. APPPS1;LMP7^+/+: p = 0.0001; IL-10: LMP7^+/+ vs. APPPS1;LMP7^+/+: p < 0.0001; LMP7^−/− vs. APPPS1;LMP7^+/+: p < 0.0001; APPPS1;LMP7^−/− vs. APPPS1;LMP7^+/+: p = 0.0002; one-way ANOVA followed by Bonferroni post-tests)