Fig. 3
α-Syn Expression in NHPs Following injection of Olig001-α-syn. Representative a LB509 and b phosphorylated serine-129 stained image striatal sections of nonhuman primate brains 3-months following administration of Olig001-α-syn (scale bar 5 mm) . α-syn is seen throughout the striatum in white matter tracts (high magnification, scale bar 100 μm, 25 μm) c Quantification using unbiased stereological counts of pSer-129 inclusions estimates 2,606,199.5 ± 322,082.98 pSer-129+ aggregates, d which covered 124.8 ± 2.18 mm3 of the striatum. e pS129 staining with and without proteinase K digestion indicates the formation of insoluble aggregates throughout the striatum (scale bar 30 μm). f Confocal microscopy confirms the cytoplasmic localization of α-syn in oligodendrocytes, shown by LB509 immunoreactivity (red channel) wrapped around nuclear Olig2 signal (green channel) (scale bar 300 μm)