Fig. 2
From: Evaluation of a novel antibody to define histone 3.3 G34R mutant brain tumours
(Left) ELISA showing reactivity of crude antisera (black), unbound fraction after affinity enrichment step (red), and purified antibodies in glycine (blue) and TEA (green) elutions, against antigenic peptide (top, G34V) or the wild-type histone sequence (below). (Right) Western blot showing purified recombinant GST-histone proteins as indicated are selectively detected with different antibodies. H3-G34R (1/250) and H3-G34V (1/500) are antibodies generated in this study; H3-K27M (1/1000) and H3 wild-type (WT, 1/2000) are commercially available