Fig. 5

Verification of ROCK2 as the novel autoantigen by indirect immunofluorescence. a: Indirect immunofluorescence using acetone-fixed ROCK2- or mock-transfected HEK293 cells incubated with patient’s serum, control serum (each 1:320) or patient’s CSF (1:10) (green). Scale bar = 50 μm; all figures same magnification. b: Neutralisation of immunofluorescence reaction on cerebellum rat and ROCK2-transfected HEK293 cells. Patient serum (green) was pre-incubated with extracts of HEK293 cells transfected with ROCK2 or with empty vector as control. The extract containing ROCK2 abolished the immune reaction. Nuclei were counterstained by incubation with TO-PRO-3 iodide (blue). Inserts show enlargement of positive and negative ROCK2-transfected cells. Scale bar = 100 μm