Fig. 1
From: Brain-derived exosomes from dementia with Lewy bodies propagate α-synuclein pathology
Isolation of exosomes from patient brain tissue. a Schematic of ultracentrifugation protocol used to isolate exosomes from tissue. Note that the pellet is the “exosome fraction”, and the supernatant is the “control fraction”. b Exosome identification markers present in the exosome fraction of Ctl, AD and DLB brain tissue. c Top Row: Representative EM micrographs from control fraction as well as Ctl, AD and DLB exosome fractions. Bottom Row: EM micrographs from control fraction and Ctl, AD and DLB exosome fractions stained with immunogold labeled anti-CD 63. Scale bar = 50 nm