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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Myelin-specific multiple sclerosis antibodies cause complement-dependent oligodendrocyte loss and demyelination

Fig. 3

MS#30 and NMO rAb #53 (NMO#53) induce demyelination at different rates. Cerebellar slices were treated with MS#30 + HC (ad, m), or NMO#53 + HC (eh, n), or Iso + HC (il, o). At the indicated time points, slices were fixed and stained with MBP (red) and NF-H (blue) antibodies. Confocal images were taken with 25X (al) or 63X (mo) objectives. The coverage of MBP on NF-H+ axons was quantified using a Matlab algorithm and normalized by control (Iso + HC treated slices) at 8 h (p). Statistical analyses were performed by multiple unpaired Student’s t test. *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, ns: not significant, n = 3-4. Confocal images of MBP and NF-H stained slices treated with rAbs alone at 48 h (qs). Scale bars: 100 μm

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