Fig. 6
Age-dependent dysfunction of VMAT2 expression and function in G2019S knock-in (KI) mice. Motor activity in 12-month-old G2019S KI mice and wild-type (WT) littermates treated with reserpine (1 mg/kg, i.p.) or saline, and challenged in the bar (a), drag (b) and rotarod (c) tests, before (baseline) and after (24 and 48 h) drug administration. Motor performance was expressed as percentage of performance at baseline. Data are means ± SEM of n = 14–15 mice per group and were analyzed using conventional ANOVA followed by the Newman–Keuls test for multiple comparisons. # p < 0.05, ## p < 0.01 significantly different from saline. Kinetic analysis of [3H]-DA uptake in whole-brain vesicles and Western blotting of VMAT2 levels in the striata from 12-month-old (d-f) or 3-month old (g- i) G2019S KI mice and WT littermates. In Western blotting, two different anti-VMAT2 antibodies were used, one commercially available (e, h; Sigma) and another developed by Miller lab (f, i) (see Methods). Data are expressed as mean ± SEM of 4 mice (d- f), 3 mice (g) or 5 mice (h, i) per group, performed in duplicate. Statistical analysis was performed by the Student t-test, two-tailed for unpaired data. **p < 0.01, different from WT