Fig. 3

Viable microglia yield is correlated with CSF pH, not age or PMD. a-c Scatterplots showing the distribution of age, PMD, and CSF pH across donor groups. The MS donor group shows significant differences in both age and PMD compared to other groups (one way ANOVA, Dunn’s multiple comparison test). Note that CSF pH is not related to neurological diagnosis. d The number of microglia isolated per gram tissue is higher in WM compared to GM isolations (unpaired Mann-Whitney test). Isolations performed using the previous method are denoted in red, those using the current method in blue, continued in following graphs. e-f Microglia yield per gram of WM or GM tissue from different neurological groups shows no differences due to diagnosis (one way ANOVA, Dunn’s multiple comparison test). g-i Microglia yield from WM tissue shows a significant positive correlation with CSF pH, but not with PMD or age (Spearman correlation). j-l Microglia yield from GM tissue shows a significant positive correlation with CSF pH, but not with PMD or age (Spearman correlation). *p value <0.05, **p value < 0.01, ***p value < 0.001, ****p value < 0.0001